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Chemiluminescence of Luminol

Investigators often find bloodstains during their examination of a crime scene. They also find similar stains that could be a different substance, something other than blood, such as red paint. How would you test a stain to see if it is blood?

Human blood contains a pigment called haemoglobin, which is used to transport oxygen through our body. This pigment is used in a number of tests to identify the presence of blood. The most common test used by investigators to reveal the presence of blood is the Luminol Test. In this test the bloodstain can be made to glow with a blue light due to the chemiluminescent reaction of the Luminol reagent with the iron (Fe) in the haemoglobin.

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View and/or download the Datadisc file examples of this investigation

initial luminescence

luminescence after adding the 'refreshing' iron

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